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Article | Tropical Aquatic and Soil Pollution | Volume 3 - Issue 1 - 2023 | 24-35 | https://doi.org/10.53623/tasp.v3i1.132
© 2023 by the author(s), and is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) License Creative Commons Attribution 4.0 International (CC BY 4.0) License

Evaluation of the Impact of Crude Oil Contamination on Soil's Physicochemical Characteristics, Micro-flora and Crop Yield

Author(s): Ugochukwu Chukwuma Okafor
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Applied Microbiology and Brewing Department, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria.

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Tropical Aquatic and Soil Pollution

Volume 3 - Issue 1 - 2023

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The effects of crude oil pollution on soil physicochemical properties, microflora, and ecotoxicity were evaluated. Soil samples were contaminated with crude oil, and the effects of contamination on the physicochemical parameters, microflora, and growth index of bean (Phaseolus vulgaris) seeds were studied over a 6-month period. The heterotrophic bacteria isolated from the uncontaminated soil were Micrococcus, Klebsiella, Flavobacterium, Bacillus, Pseudomonas, and Serratia species, and the moulds included microbes such as Aspergillus niger, Fusarium, and Mucor sp. Petroleum contamination increased the pH of the soils to alkaline values while increasing the total nitrogen, organic carbon, and phosphorus contents. Electrical conductivity, nitrogen content, and phosphorus content were significantly reduced after petroleum contamination (p ˂ 0.05). The heavy metal contents of the contaminated soils decreased with increasing remediation time. Zinc, total nitrogen, total organic carbon, and electrical conductivity contents were statistically significantly different among samples throughout the bioremediation period (p ˂ 0.05). The ability of isolates to utilise hydrocarbons was highest for Pseudomonas and Bacillus species and lowest for Klebsiella and Serratia species. After a germination period of 12 days, a germination test showed that the bioattenuated polluted soil improved germination of bean seeds. Bioattenuation methods should be used and improved as a means of remediating petroleum-polluted sites because they are cost-effective and environmentally friendly.
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Akiner, S. (2006). The Caspian: politics, energy and security, 1st ed; Routledge: London, United Kingdom.

Okoh, A.L.; Trejo-Hernandez. M.R (2006) Remediation of Petroleum Hydrocarbon polluted systems: Exploiting the Bioremediation strategies. African Journal of Biotechnology, 5, 2520-2525. https://doi.org/10.5897/AJB2006.000-5108.

Ekpo, M.A.; Udofia, U.S. (2008). Rate of Biodegradation of Crude Oil by Microorganisms isolated from Oil Sludge Environment. African Journal of Biotechnology, 7, 4495-4499. https://doi.org/10.5897/AJB08.886.

Brooke, A.A.; Itah, A.Y. (2004). Fusion of two yeast strains for enhanced crude oil degradation. Global Journal of Pure and Applied Sciences, 10, 491-496. http://doi.org/10.4314/gjpas.v10i4.16428 .

Okieimen, C.O.; Okieimen, F.E. (2005), Bioremediation of crude oil polluted soil-effect of Poultry Droppings and Natural Rubber Processing Sludge: Application on Biodegradation of Petroleum Hydrocarbons. Environmental Sciences, 1, 1-8. https://doi.org/10.1016/S0960-8524(01)00157-2.

Azubuike, C.C.; Chikere, C.B.; Okpokwasili, G.C. (2016). Bioremediation techniques- Classification based on Site of Application: Principles, Adavantages, Limitations and Prospects. World Journal of Microbiology and Biotechnology, 32, 180. https://doi.org/10.1007/s11274-016-2137-x.

Adenipekun, C.O. (2008). Bioremediation of engine-oil polluted soil by Pleurotus tuberegium, a Nigerian White-rot Fungus. African Journal of Biotechnology, 7, 55-58.

Garcı´a-Delgado, C.; Alfaro-Barta, I.; Eymar, E. (2015).Combination of Biochar Amendment and Mycoremediation for Polycyclic Aromatic Hydrocarbons Immobilization and Biodegradation in Creosote-contaminated Soil. Journal of Hazardous Materials, 285, 259–266. https://doi.org/10.1016/j.jhazmat.2014.12.002.

Sardrood, B.P.; Goltapeh, E.M.; Varma, A. (2013). An Introduction to Bioremediation. In Fungi as Bioremediators, Soil Biology; Goltapeh, E., Danesh, Y., Varma, A., Eds.; Springer, Berlin, Germany; Volume 32, pp 1-27. http://doi.org/10.1007/978-3-642-33811-3_1.

Loredana, S.; Roberto, S.; Margharita, L.; Emanuela, A.; Adriana, G. (2010). Bioremediation of bacteria in aquaculture waste using the polychaete Sabella spallanzanii. New Biotechnology, 24, 43-47. https://doi.org/10.1016/j.nbt.2010.06.018.

Jacoby, R.; Peukert, M.; Succurro, A.; Koprivova, A.; Kopriva, S. (2017). The Role of Soil Microorganisms in Plant Mineral Nutrition-Current Knowledge and Future Directions. Frontiers in Plant Science, 8, 1617. https://doi.org/10.3389/fpls.2017.01617.

Okafor U.C.; Umeneanya D.N.; Umeozor F.O. (2022). Bioload, Ecotoxicity and Physicochemical Evaluation of Paint Industries’ Effluent Contaminated Sites in Aba, Abia State, Nigeria. Asian Journal of Plant and Soil Sciences, 7, 277-287.

Okafor, U.C.; Orji, M.U.; Agu, K.C.; Awah, N.S.; Okeke, B.C.; Okafor, O.I.; Okoro, N.C.N (2016). Bioremediation of Crude Oil-polluted Soil Using Broiler-Chicken droppings. Journal of Applied and Environmental Microbiology, 4, 75-84. http://doi.org/10.12691/jaem-4-4-2.

Ibe, N.; Ibe. E.G. (1985). Control and Dispersion Potential of oil Spills by Bacterial Seeding, In The Petroleum Industry and the Nigeria Environment. Proceedings of an International Warn. Nigeria; pp. 112-115.

Okafor, U.C.; Orji M.U.; Nwankwegu A.S.; Anaukwu C.G.; Onuorah S.C.; Archibong E.J.; Obika I.E.; Agu K.C. (2016). Effect Of Chicken Droppings Amendment on Bioremediation of Crude Oil Polluted Soil. European Journal of Experimental Biology, 6, 62-68.

Holt, J.G.; Krieg, N.R.; Sneath, P.H.A.; Stanley, J.T.; William, S.T. (1994) Bergey’s Manual of Determinative Bacteriology. Lippincott Williams & Wilkins: Baltimore, USA.

Okpokwasili, G.C.; Amanchukwu, S.C. (1988). Petroleum Hydrocarbon Degradation by Candida species. Environmental International, 14, 234-247. https://doi.org/10.1016/0160-4120(88)90145-6.

Collins, C.H.; Lyne, P.M. (1980). Microbiological methods. Butterworths: London, UK.

Riskuwa-Shehu, M.L.; Ijah, U.J.J. (2016). Enhanced Removal of Crude Oil in Soil by Mixed Culture of Bacillus Megaterium UL05 and Pseudomonas Aeruginosa UL07. International Journal of Environmental Bioremediation & Biodegradation. 4, 8-12. https://doi.org/10.12691/ijebb-4-1-2.

Ijah, U.J.; Antai, S.P. (2003). The Potential use of chicken droppings microorganisms for oil spill remediation. The Environmentalist, 23, 89-95. https://doi.org/10.1023/A:1022947727324.

American Public Health Association (1998). Standard Methods for the Examination of Water and Wastewater, 20th Edition; APHA: Washington DC: USA.

Dagona, G.A. (2012). Bioremediation of textile industries effluents using selected Bacterial species in Kano, Nigeria. Master Thesis, Ahmadu Bello University, Nigeria.

Okafor U.C.; Orji M.U.; Umeh S.O.; Onuorah S.C. (2022). Effects of Effluents’ Discharge from Some Paint Industries on Soil’s Physicochemical Properties and Bioattenuation of Polluted Soil. Industrial and Domestic Waste Management, 2, 46-60. https://doi.org/10.53623/idwm.v2i2.110.

Kalra, Y.P. (1996). Soil pH: First soil analysis methods validated by the AOAC International. Journal of Forest Research, 1, 61–64. https://doi.org/10.1007/BF02348343.

Yakubu, M.J. (2007). Biodegradation of Lagoma Crude Oil using Pig Dung. African Journal of Biotechnology, 6, 2821-2828. https://doi.org/10.5897/AJB2007.000-2450.

Millioli V.S.; Servuloa, E.F.C.; Sobral, L.G.S.; de Carvalhoa, D.D. (2009). Bioremediation of crude oil-bearing soil: Evaluation of rhamnolipid addition as for the toxicity and crude oil biodegradation efficiency. Global Nest Journal, 11, 181-188. https://doi.org/10.30955/gnj.000592.

Okafor, U.C.; Nwankwegu A.S. (2016). Effect of Woodchips on Bioremediation of Crude Oil polluted Soil. British Microbiology Research Journal, 15, 1-7. http://doi.org/10.9734/BMRJ/2016/27027.

Okafor U.C.; Nwose O.D. (2018). Assessment of Hydrocarbon-Utilizing Microorganisms from Spent Engine Oil-Polluted Soils from Mechanic Workshops in Awka Metropolis. International Journal of Chemical and Biomolecular Science, 4, 33-40.

Okafor UC, Orji MU. (2022). Remediation potentials of wheat-bran and wood-chips on effluent contaminated soils from paint industries in Aba, Abia State Nigeria. Journal of Global Ecology and Environment, 15, 1-17.

Mara, D.D.; Kramer, A. (2008) The 2006 WHO Guidelines for Wastewater and Greywater Use in Agriculture: A Practical Interpretation. In Efficient Management of Wastewater: Its Treatment and Reuse in Water Scarce Countries; Al Baz, I., Otterpohl, R., Wendland, C., Eds.; Springer: Heidelberg, Germany, pp. 1-17.

Michael, O.A.; Romeo, C.O.; Daniel, I.O. (2017). Physicochemical Parameters and Heavy Metals Assessment of Effluent Discharges from Some Industries in Benin City, Nigeria. African Scientist, 18, 183-187.

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SUBMITTED: 16 October 2022
ACCEPTED: 02 January 2023
PUBLISHED: 7 January 2023
SUBMITTED to ACCEPTED: 78 days
DOI: https://doi.org/10.53623/tasp.v3i1.132

Cite this article
Okafor, U. C. (2023). Evaluation of the Impact of Crude Oil Contamination on Soil’s Physicochemical Characteristics, Micro-flora and Crop Yield. Tropical Aquatic and Soil Pollution, 3(1), 24–35. https://doi.org/10.53623/tasp.v3i1.132
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